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Phase optimized liquid chromatography as an instrument for steroid analysis
Author(s) -
Kuehnle Maximilian,
Rehbein Jens,
Holtin Karsten,
Dietrich Benjamin,
Gradl Manuela,
Yeman Helen,
Albert Klaus
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700604
Subject(s) - selectivity , chromatography , stationary phase , phase (matter) , chemistry , reversed phase chromatography , steroid , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , biochemistry , hormone , catalysis
A new method using phase optimized LC (POPLC) for the analysis of steroids is described. The retention factors and the theoretical plate numbers of different steroids were determined for four different stationary phases. Based on these values, an optimal stationary phase composition and the resultant chromatogram have been calculated by an optimization software and compared to the measured data. It is demonstrated that these predicted data show only little differences to the experimental results. Furthermore, it is shown that the overall selectivity of the optimal stationary phase composition is much better than the selectivity of any individual stationary phase.