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Separation and determination of enkephalin‐related peptides by capillary electrophoresis with amperometric detection
Author(s) -
Wang Wei,
Yao Sheyue,
Qiu Bin,
Chen Guonan
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700538
Subject(s) - amperometry , capillary electrophoresis , chromatography , chemistry , enkephalin , detection limit , phosphate buffered saline , methanol , analytical chemistry (journal) , electrode , electrochemistry , biochemistry , receptor , organic chemistry , opioid
A simple, reliable, and reproducible method for separation and determination of five enkephalin‐related peptides based on CE with amperometric detection (AD) is described in this paper. A potential of 1.0 V was applied to the carbon disk electrode, which was used as a working electrode in this system. At 15 kV of applied voltage, the five compounds were separated within 18 min in a 20 mmol/L phosphate buffer solution (PBS, pH 7.6) including 2.5% methanol v/v. LOD for five enkephalins were ranged from 6.31 to 54.3 nmol/L. The method was applied successfully to determine the five compounds added in the human cerebrospinal fluid (CSF) and the recoveries were in the range of 95.8–98.2%.