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Stability‐indicating assay of sodium cromoglicate in ophthalmic solution using mixed‐mode hydrophilic interaction chromatography
Author(s) -
Ali Mohammed Shahid,
Rafiuddin Syed,
AlJawi Dalya Abdullah,
AlHetari Yasmin,
Ghori Mohsin Ul Haq,
Khatri Aamer Roshanali
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700534
Subject(s) - hydrophilic interaction chromatography , chemistry , chromatography , solvent , high performance liquid chromatography , sodium , ionic strength , aqueous solution , organic chemistry
A hydrophilic interaction chromatographic (HILIC) procedure for the quantification of Sodium Cromoglicate (SCG) in ophthalmic solution is developed. Mobile phase consists of ACN and buffer, 86:14 v/v. Atlantis HILIC–Si column, 25 cm×4.6 mm, is used as stationary phase. Detection is carried out using a variable wavelength UV‐Vis detector at 326 nm. Linearity range and percent recoveries for SCG were 50–400 μg/mL and 100.44%, respectively. The SCG HILIC‐UV assay was validated according to the International Conference on Harmonization guidelines. The method separates two impurities and degradation products resulting from stress environment. Influence of organic solvent, ionic strength and mobile phase pH on the retention of SCG is studied. The paper provides optimization of polar anionic solute (SCG) on unmodified silica by HILIC. Proposed method can be used as a stability‐indicating assay for SGC and can be proved to be beneficial in ESI‐MS for enhanced sensitivity.