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On‐line concentration and pressurized capillary electrochromatographic analysis of phytohormones in corn
Author(s) -
Wang Shujuan,
Jia Li,
Xing Da,
Chen Deping,
Zhao Jingshan
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700519
Subject(s) - chromatography , chemistry , stacking , electrolyte , solvent , detection limit , analytical chemistry (journal) , absorbance , electrode , organic chemistry
A pressurized CEC (pCEC) method was developed for the separation of phytohormones, in which UV absorbance was used as the detector and a monolithic silica‐ODS column as the separation column. The parameters (including the concentration of organic solvent in the mobile phase, pH of the electrolyte buffer, applied voltage) affecting the separation resolution were evaluated. Two on‐line concentration techniques, namely, solvent gradient zone sharpening effect and field‐enhanced sample stacking, were utilized to improve detection sensitivity. The combination of the two techniques proved to be beneficial to enhance the detection sensitivity by enabling the injection of large volumes of samples. Compared to the conventional injection mode, the enhancement in the detection sensitivities of phytohormones using the on‐line concentration technique is in the range from 9‐ to 23‐fold. The developed pCEC method was applied to evaluate phytohormones in corns.