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Quantitative determination of amino acids in functional foods by microchip electrophoresis
Author(s) -
Ueno Hiroko,
Wang Jun,
Kaji Noritada,
Tokeshi Manabu,
Baba Yoshinobu
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700517
Subject(s) - chromatography , chemistry , amino acid , micellar electrokinetic chromatography , quantitative analysis (chemistry) , capillary electrophoresis , electrophoresis , fluorescence , starch , biochemistry , physics , quantum mechanics
Microchip electrophoresis (MCE), a first‐generation micrototal analysis system, has emerged during the miniaturization phase of food analysis. Based on the micellar electrokinetic chromatography mode, a simple and fast MCE method with light emitting diode‐induced fluorescence detection was developed for quantitative analysis of amino acids in three different kinds of functional foods, viz. sports beverages, jelly‐form beverages, and tablet‐form functional foods. In contrast to the glass microchip, we improved the separation of amino acids on a poly(methyl methacrylate) (PMMA) chip by addition of cationic starch derivatives. 4‐Fluoro‐7‐nitro‐2,1,3‐benzoxadiazole, which has a short labeling time for amino acids, was used as the fluorescently labeled dye. This MCE method takes less than 10 min of total analysis time including sample preparation and analysis of amino acids in functional foods on a PMMA chip. The results show that this approach has the potential to be a fast and simple method for amino acid analysis in functional foods.