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S ‐Trityl‐( R )‐cysteine, a powerful chiral selector for the analytical and preparative ligand‐exchange chromatography of amino acids
Author(s) -
Natalini Benedetto,
Sardella Roccaldo,
Macchiarulo Antonio,
Pellicciari Roberto
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700511
Subject(s) - chemistry , enantiomer , resolution (logic) , chromatography , ligand (biochemistry) , amino acid , chiral stationary phase , cysteine , salt (chemistry) , ion chromatography , organic chemistry , biochemistry , receptor , artificial intelligence , computer science , enzyme
S ‐Trityl‐( R )‐cysteine [( R )‐STC] is the new selector of a dynamically coated, chiral ligand‐exchange stationary phase which proved to be highly effective in both analytical and preparative‐scale separation of enantiomers of some natural and unnatural underivatized amino acids, with good separation and resolution factors. With the aim of identifying the best chromatographic conditions suitable for the preparative‐scale separations, some parameters controlling retention, separation and resolution factors (such as the type and amount of cupric salt and the eluent pH) were investigated. The relatively easy removal of the Cu(II) ions renders this technique suitable for obtaining small amounts of enantiomerically pure samples for preliminary biological evaluations.