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Screening of anti‐platelet aggregation agents from Panax notoginseng using human platelet extraction and HPLC–DAD–ESI‐MS/MS
Author(s) -
Wang Jing,
Huang Zhaogang,
Cao Hui,
Wang Yitao,
Hui Ping,
Hoo Chai,
Li Shaoping
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700507
Subject(s) - panax notoginseng , chemistry , adenosine diphosphate , high performance liquid chromatography , platelet , thrombin , ginsenoside , arachidonic acid , platelet aggregation , guanosine , chromatography , adenosine , pharmacology , extraction (chemistry) , traditional medicine , biochemistry , medicine , ginseng , enzyme , immunology , alternative medicine , pathology
Root of Panax notoginseng ( Sanqi in Chinese) is a highly valued and commonly used Chinese medicine. It has been widely used for treatment of cardio‐ and cerebro‐vascular diseases. In this study, a method involving human platelet extraction and HPLC–DAD–ESI‐MS/MS was developed for screening potential anti‐platelet aggregation agents in Sanqi . Five compounds which could interact with human platelets were found, and four were identified as adenosine, guanosine, ginsenoside Rh1, and ginsenoside F1, respectively. The effects on rabbit platelet aggregation induced by arachidonic acid, adenosine diphosphate, and thrombin were also investigated in vitro . The results showed that the nucleosides adenosine and guanosine mainly contributed to the anti‐platelet aggregation of Sanqi . The data suggest that human platelet extraction combined with HPLC–DAD–ESI‐MS/MS is a useful method for screening anti‐platelet aggregation agents from Chinese medicines.