Premium
Determination of estrogens in wastewater using three‐phase hollow fiber‐mediated liquid‐phase microextraction followed by HPLC
Author(s) -
Liu Meihua,
Qiu Bin,
Jin Xia,
Zhang Lan,
Chen Xi,
Chen Guonan
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700497
Subject(s) - chromatography , chemistry , high performance liquid chromatography , extraction (chemistry) , hexestrol , detection limit , phase (matter) , fiber , wastewater , sodium hydroxide , enrichment factor , diethylstilbestrol , organic chemistry , biochemistry , hormone , engineering , waste management
Three‐phase hollow fiber‐mediated liquid‐phase microextraction followed by HPLC was used for the determination of three synthetic estrogens, namely diethylstilbestrol, dienestrol, and hexestrol, in wastewater. Extraction conditions including organic solvent, volume ratio between donor solution and acceptor phase, extraction time, stirring rate, donor phase and acceptor phase were optimized. The target compounds were extracted from a 10 mL aqueous sample at pH 1.5 (donor solution) through a 45 mm in length hollow polypropylene fiber that was immersed in 1‐octanol in advance, and then the hollow fiber was filled with 10 μL 0.5 mol/L sodium hydroxide solution (acceptor phase). After a 40 min extraction, the acceptor phase was directly injected into an HPLC system for detection. Under the optimized extraction conditions, a large enrichment factor (more than 300‐fold) was achieved for the three estrogens. The determination limit at an S/N of 3 ranged from 0.25 to 0.5 μg/L for the estrogens. The recovery ratio was more than 86% in the determination of these estrogens in wastewater.