6‐Oxy‐(acetyl ethylenediamine) fluorescein, a novel fluorescent derivatization reagent for carboxylic acids and its application in HPLC | Zendy

Du XiaoLan | Zendy; Wang Hong | Zendy; Ma Ming | Zendy; Deng YingHua | Zendy; Zhang HuaShan | Zendy

Premium

6‐Oxy‐(acetyl ethylenediamine) fluorescein, a novel fluorescent derivatization reagent for carboxylic acids and its application in HPLC

Author(s) -

Du XiaoLan,

Wang Hong,

Ma Ming,

Deng YingHua,

Zhang HuaShan

Publication year - 2008

Publication title -

journal of separation science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.72

H-Index - 102

eISSN - 1615-9314

pISSN - 1615-9306

DOI - 10.1002/jssc.200700456

Subject(s) - chemistry , derivatization , chromatography , carboxylic acid , reagent , high performance liquid chromatography , hexanoic acid , ethylenediamine , lauric acid , organic chemistry , oleic acid , palmitic acid , fatty acid , biochemistry

A novel fluorescent derivatization reagent for carboxylic acids, 6‐oxy‐(acetyl ethylenediamine) fluorescein (AEF), was well designed, synthesized, and applied to HPLC. The derivatization reaction with 12 fatty acids, including n ‐valeric acid (C5), n ‐hexanoic acid (C6), n ‐heptanoic acid (C7), n ‐octanoic acid (C8), n ‐nonanoic acid (C9), n ‐decanoic acid (C10), lauric acid (C12), myristic acid (C14), palmitic acid (C16), stearic acid (C18), oleic acid (C18:1), and linoleic acid (C18:2), was completed at 55°C within 40 min. The derivatives of fatty acids were separated on a C18 RP column and detected by fluorescence detection. The LODs attained were 0.4–1.2 nM (S/N of 3). It has been demonstrated that AEF is a prominent derivatization reagent for carboxylic acids which is suitable for HPLC.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research