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6‐Oxy‐(acetyl ethylenediamine) fluorescein, a novel fluorescent derivatization reagent for carboxylic acids and its application in HPLC
Author(s) -
Du XiaoLan,
Wang Hong,
Ma Ming,
Deng YingHua,
Zhang HuaShan
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700456
Subject(s) - chemistry , derivatization , chromatography , carboxylic acid , reagent , high performance liquid chromatography , hexanoic acid , ethylenediamine , lauric acid , organic chemistry , oleic acid , palmitic acid , fatty acid , biochemistry
A novel fluorescent derivatization reagent for carboxylic acids, 6‐oxy‐(acetyl ethylenediamine) fluorescein (AEF), was well designed, synthesized, and applied to HPLC. The derivatization reaction with 12 fatty acids, including n ‐valeric acid (C5), n ‐hexanoic acid (C6), n ‐heptanoic acid (C7), n ‐octanoic acid (C8), n ‐nonanoic acid (C9), n ‐decanoic acid (C10), lauric acid (C12), myristic acid (C14), palmitic acid (C16), stearic acid (C18), oleic acid (C18:1), and linoleic acid (C18:2), was completed at 55°C within 40 min. The derivatives of fatty acids were separated on a C18 RP column and detected by fluorescence detection. The LODs attained were 0.4–1.2 nM (S/N of 3). It has been demonstrated that AEF is a prominent derivatization reagent for carboxylic acids which is suitable for HPLC.