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Recognition of Staphylococcus enterotoxin via molecularly imprinted beads
Author(s) -
Yao Wei,
Ning Baoan,
Zhou Huanying,
Fang Yanjun,
Gao Zhixian
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700429
Subject(s) - molecularly imprinted polymer , adsorption , chemistry , molecular imprinting , selective adsorption , polystyrene , bead , imine , enterotoxin , polymer , covalent bond , polymerization , chromatography , polymer chemistry , chemical engineering , nuclear chemistry , selectivity , organic chemistry , materials science , catalysis , biochemistry , escherichia coli , engineering , composite material , gene
The synthesis of molecularly imprinted beads for the recognition of the protein Staphylococcus enterotoxin B (SEB) is described. Two kinds of organic silane (3‐aminopropyltrimethoxysilane (APTMS) and octyltrimethoxysilane (OTMS)) were polymerized on the surface of polystyrene microspheres after the SEB template was covalently immobilized by forming imine bonds. The resulting imprinted beads were selective for SEB. The Langmuir adsorption models were applied to describe the equilibrium isotherms. The results showed that an equal class of adsorption was formed in the molecularly imprinted polymer (MIP) with the maximum adsorption capacity of 3.86 mg SEB/g imprinted beads. The MIP has much higher adsorption capacity for SEB than the nonimprinted polymer, and the MIP beads have a higher selectivity for the template molecule.