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Chemical stability of haloperidol injection by high performance thin‐layer chromatography
Author(s) -
Mennickent Sigrid,
Pino Loreto,
Vega Mario,
de Diego Marta
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700404
Subject(s) - chromatography , chemistry , ampoule , silica gel , acetic acid , high performance thin layer chromatography , haloperidol , thin layer chromatography , high performance liquid chromatography , acetone , chloroform , analytical chemistry (journal) , biochemistry , neuroscience , biology , dopamine , organic chemistry
The chemical stability of haloperidol lactate injection was studied under different storage conditions by high performance thin‐layer chromatography (HPTLC). The study was performed at 25 ± 2°C and at refrigeration temperature (8 ± 1°C) in original glass ampoules over 15 days after being opened. The samples tested at 25 ± 2°C were stored with exposure to and protection from light. Chromatographic separation was achieved on precoated silica gel F 254 HPTLC plates using a mixture of acetone/chloroform/ n ‐butanol/glacial acetic acid/water (5:10:10:2.5:2.5, v/v/v/v/v) as a mobile phase. Quantitative analyses were carried out at a wavelength of 254 nm. The method exhibited adequate linearity ( r = 0.999), selectivity, precision (RSD = 1.92%), and accuracy (recoveries from 98.59 to 101.90%). The concentrations of all samples remained greater than or 90% of the original concentration. Haloperidol lactate injection was chemically stable under all conditions studied over 15 days.