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Preparation and evaluation of rigid porous polyacrylamide‐based strong cation‐exchange monolithic columns for capillary electrochromatography
Author(s) -
Dong Jing,
Ou Junjie,
Dong Xiaoli,
Wu Renan,
Ye Mingliang,
Zou Hanfa
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700402
Subject(s) - capillary electrochromatography , monolith , chemistry , monolithic hplc column , electrochromatography , chromatography , monomer , sulfonic acid , dodecanol , phase (matter) , polymerization , capillary electrophoresis , high performance liquid chromatography , polymer , polymer chemistry , organic chemistry , catalysis
A CEC monolithic column with strong cation‐exchange (SCX) stationary phase based on hydrophilic monomers was prepared by in situ polymerization of acrylamide, methylenebisacrylamide, and 2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid (AMPS) in a complete organic binary porogenic solvent consisting of DMSO and dodecanol. The sulfonic groups provided by the monomer AMPS on the surface of the stationary phase generate an EOF from anode to cathode, and serve as an SCX stationary phase at the same time. The monolithic stationary phase exhibited normal‐phase chromatographic behavior for neutral analytes. For charged analytes, electrostatic interaction/repulsion with the monolith was observed. The strong SCX monolithic column has been successfully employed in the electrochromatographic separation of basic drugs, peptides, and alkaloids extracted from natural products.