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Two‐dimensional LC–MS analysis of components in Swertia franchetiana Smith
Author(s) -
Liu Yanming,
Xu Qing,
Xue Xingya,
Zhang Feifang,
Liang Xinmiao
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700393
Subject(s) - chromatography , chemistry
An off‐line 2‐D‐LC approach was developed for the separation and identification of components in extracts of Swertia franchetiana Smith, which is a traditional Chinese medicine. An octadecylsilica column was used for further separation of fractions from a cyano column with UV and MS detection. In this method, a longer column was used in the second dimension to improve the separation ability, and “on‐column focusing” was adopted to reduce sample loss and pollution on concentrating the fractions from the first dimension. More than 118 and 575 components were detected in the chloroform and n ‐butanol extracts by this method, respectively. In contrast, only 20 and 95 peaks were detected by the one‐dimensional method. Most of the additionally detected compounds were of low‐abundance components. Many compounds can be preliminarily identified according to their UV and mass spectra in the two‐dimensional method. The obtained results not only demonstrated the powerful resolution of 2‐D‐LC, but also led to in‐depth comprehension of the UV and mass spectra of complex samples.