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Diagnosis of Helicobacter pylori infection with the 13 C‐urea breath test by means of GC‐MS analysis
Author(s) -
Jordaan Maraliese,
Laurens Johannes B.
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700385
Subject(s) - breath test , helicobacter pylori , predictive value , gold standard (test) , urea breath test , helicobacter pylori infection , gastroenterology , chemistry , medicine
Abstract The diagnosis of Helicobacter pylori (H. pylori) infection by GC‐MS detection of the 13 CO 2 enrichment in 13 C‐urea breath test ( 13 C‐UBT) samples is reported. This study aimed to optimize the 13 C‐UBT with regards to the diagnostic cut‐off value, sampling time, and frequency. The H. pylori status of 103 dyspeptic patients was obtained by histological examination, the rapid urease test as well as with the GC‐MS 13 C‐UBT. Analytical and diagnostic accuracies were determined by comparison of the GC‐MS 13 C‐UBT results with that of the analytical and diagnostic gold standards, namely GC‐isotope ratio MS (IRMS) and histology. The 13 CO 2 enrichment values obtained with GC‐MS analysis, correlated favorably ( r 2 = 0.993) with those obtained by GC‐IRMS analysis. When compared to histology, the GC‐MS 13 C‐UBT had a diagnostic sensitivity of 92% and a specificity of 93%. The positive predictive value (PPV), negative predictive value (NPV), and accuracy were 95, 89, and 92%, respectively. It was concluded that SIM GC‐MS is capable of analyzing nonradioactive 13 C‐UBT samples, with a precision and accuracy sufficient to distinguish between H. pylori positive and negative patients.