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Quality evaluation of Yin Chen Hao Tang extract based on fingerprint chromatogram and simultaneous determination of five bioactive constituents
Author(s) -
Wang Xijun,
Lv Haitao,
Sun Hui,
Jiang Xingang,
Wu Zeming,
Sun Wenjun,
Wang Ping,
Liu Lian,
Bi Kaishun
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700376
Subject(s) - chromatography , chemistry , fingerprint (computing) , high performance liquid chromatography , analyte , chromatographic separation , artificial intelligence , computer science
Abstract A completely validated method based on HPLC coupled with photodiode array detector (HPLC‐UV) was described for evaluating and controlling quality of Yin Chen Hao Tang extract (YCHTE). First, HPLC‐UV fingerprint chromatogram of YCHTE was established for preliminarily elucidating amount and chromatographic trajectory of chemical constituents in YCHTE. Second, for the first time, five mainly bioactive constituents in YCHTE were simultaneously determined based on fingerprint chromatogram for furthermore controlling the quality of YCHTE quantitatively. The developed method was applied to analyze 12 batches of YCHTE samples which consisted of herbal drugs from different places of production, showed acceptable linearity, intraday (RSD <5%), interday precision (RSD <4.80%), and accuracy (RSD <2.80%). As a result, fingerprint chromatogram determined 15 representative general fingerprint peaks, and the fingerprint chromatogram resemblances are all better than 0.9996. The contents of five analytes in different batches of YCHTE samples do not indicate significant difference. So, it is concluded that the developed HPLC‐UV method is a more fully validated and complete method for evaluating and controlling the quality of YCHTE.