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Gradient HPLC of antibiotics in urine, ground water, chicken muscle, hospital wastewater, and pharmaceutical samples using C‐18 and RP‐amide columns
Author(s) -
Kumar Ashwini,
Kumar Malik Ashok,
Kumar Tewary Dhananjay,
Singh Baldev
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700373
Subject(s) - lomefloxacin , chromatography , nalidixic acid , chemistry , ofloxacin , high performance liquid chromatography , analyte , urine , detection limit , ciprofloxacin , antibiotics , biochemistry
A simple and highly sensitive high pressure liquid chromatographic (HPLC‐UV) method has been developed for the determination of ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid, in mobile phase citrate buffer (0.001 M) of pH 4.5 prepared in water (X), methanol (Y), and ACN (Z) using gradient at a flow rate of 1.0 mL/min by direct UV absorbance detection at λ = 280 nm. Separation of analytes was studied on the C‐18 and RP‐amide columns and best results were observed on the RP‐amide column with LODs (3.3× S / m ) 0.89, 0.55, 0.67, and 1.41 ng/mL for ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid, respectively, and better RSD than the C‐18 column. The recovery of Fluoroquinolones (FQs) in urine, ground water, hospital wastewater, and chicken muscle using this method is more than 90%. The method was successfully applied to the analysis of ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid in urine, ground water, pharmaceutical dosage forms, hospital wastewater, and chicken muscle.