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Reversed phase‐HPLC for rapid determination of polyphenols in flowers of rose species
Author(s) -
Kumar Neeraj,
Bhandari Pamita,
Singh Bikram,
Gupta Ajai P.,
Kaul Vijay K.
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700372
Subject(s) - quercitrin , chromatography , rutin , chemistry , myricetin , gallic acid , kaempferol , repeatability , quercetin , polyphenol , high performance liquid chromatography , hyperoside , catechin , chlorogenic acid , organic chemistry , antioxidant
A rapid, simple, sensitive, robust, and improved HPLC method was developed and validated for determination of 10 polyphenols, namely gallic acid, catechin, epicatechin, rutin, m ‐coumaric acid, quercitrin, myricetin, quercetin, apigenin, and kaempferol in fresh flowers of Rosa bourboniana and R. brunonii and in both fresh flowers and marc (left after industrial distillation of rose oil) of R. damascena . Six polyphenols, gallic acid, rutin, quercitrin, myricetin, quercetin, and kaempferol, were detected and quantified in all extracts. The chromatographic separation of 10 polyphenols was achieved in less than 16 min by RP‐HPLC (Phenomenex, Luna C18 (2) column, 5 μm, 250 mm×4.6 mm) using linear gradient elution of water and acetonitrile (0.02% trifluroacetic acid) with a flow rate of 1 mL/min at λ 280 nm. Standard calibration curves were linear in the range of 0.39–500 μg/mL. Good results were achieved with respect to repeatability (RSD <3%) and recovery (98.6–100.8%). The method was validated for linearity, accuracy, repeatability, LOD, and LOQ.