Premium
Separation behavior of electron‐beam curing derived, acrylate‐based monoliths
Author(s) -
Bandari Rajendar,
Elsner Christian,
Knolle Wolfgang,
Kühnel Christa,
Decker Ulrich,
Buchmeiser Michael R.
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700364
Subject(s) - curing (chemistry) , acrylate , materials science , cathode ray , separation (statistics) , electron , copolymer , polymer chemistry , composite material , polymer , computer science , physics , quantum mechanics , machine learning
Electron beam (EB) curing‐derived monolith materials were prepared from ethyl methacrylate (EMA), trimethylolpropane triacrylate (TMPTA), 2‐propanol, 1‐dodecanol, and toluene within the confines of 3 mm×100 mm id glass columns, applying a total dose of 22 kGy for curing. Monolithic columns were checked for their separation behavior for selected dansylated (DNS)‐amino acids as well as for cyclophilin 18. Their separation performance was compared to that of a C18‐modified silica‐based rigid rod (Chromoliths®). In the separation of dansylated amino acids, retention times were reduced on EB‐derived columns, where the peak resolution was significantly better than on a Chromolith®. This finding was attributed to a larger fraction of small pores (< 2.15 nm) in the EB curing‐derived monoliths. Finally, EB curing‐derived monoliths have been used to separate cyclophilin 18 from crude cell lysis mixtures.