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Monolith‐based immobilized enzyme reactors: Recent developments and applications for proteome analysis
Author(s) -
Ma Junfeng,
Zhang Lihua,
Liang Zhen,
Zhang Weibing,
Zhang Yukui
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700362
Subject(s) - proteome , monolith , chemistry , proteomics , chromatography , immobilized enzyme , biochemical engineering , computational biology , enzyme , biology , biochemistry , engineering , catalysis , gene
In the current proteome study, protein digestion is indispensable before proteins could be identified by MS/MS, no matter based on top–down or bottom–up strategies. Compared to the traditional digestion performed in free solution, immobilized enzyme shows great advantages in digestion speed, stability, and longevity, especially with monolithic materials as the supports. Besides the improved digestion capacity, the immobilized enzyme reactors (IMERs) could be further coupled with the separation and detection systems, enabling high‐throughput protein identification. In this paper, the latest advances in the monolith‐based IMERs and their applications in proteomic study are briefly summarized. By reviewing these achievements, it could be seen that monilith‐based IMERs have very bright future in proteome analysis.