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Sensitive determination of aliphatic amines by high‐performance liquid chromatography with a new fluorogenic probe 3‐(4‐fluorinebenzoyl)‐2‐quinoline carboxaldehyde
Author(s) -
Zhang Niu,
Zhao YunZhi,
Zhang HuaShan,
Wang Hong
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700341
Subject(s) - quinoline , chromatography , chemistry , high performance liquid chromatography , organic chemistry
A new fluorogenic reagent 3‐(4‐fluorinebenzoyl)‐2‐quinoline carboxaldehyde (FBQCA) has been synthesized and used as a derivatizing reagent for the determination of aliphatic amines with HPLC. The reagent is nonfluorescent, but forms highly fluorescent isoindole upon the reaction with primary amines in alkaline medium. Eleven amine derivatives were baseline separated in 8 min using a gradient elution on a C 8 column and detected with fluorescence detection at λ ex /λ em = 480/546 nm. The detection limits were in the range of 0.5–2 nM (S/N = 3). The proposed method has been successfully applied to the analysis of aliphatic amines in food and environmental samples, including white wine, soybean oil, soil, and tap water with satisfactory recoveries in the range of 94–106%.