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Determination of gentisin, isogentisin, and amarogentin in Gentiana lutea L. by capillary electrophoresis
Author(s) -
Citová Ivana,
Ganzera Markus,
Stuppner Hermann,
Solich Petr
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700325
Subject(s) - capillary electrophoresis , chromatography , chemistry , quantitative analysis (chemistry) , high performance liquid chromatography , gentiana , biology , botany
A novel, fast, and simple capillary electrophoresis method has been developed for the analysis of gentisin, isogentisin, and amarogentin in roots of Gentiana lutea (yellow gentian), an herb traditionally used as gastric stimulant. Gentisin and isogentisin are xanthones showing potent inhibition of monoamine oxidase type A and B, amarogentin represents one of the bitter principles of Gentiana, responsible for its gastric‐roborant effects. Optimal CE‐separation conditions comprise a 100 mM sodium tetraborate buffer of pH 9.3, containing 10 mM β‐cyclodextrin as additive; optimum temperature and applied voltage were found to be 30°C and 25 kV, respectively. Direct diode array detection at 260 nm (gentisin, isogentisin) and 242 nm (amarogentin) was performed, and the required analysis time was only 11 min. The developed method was validated for linearity, sensitivity, precision, and accuracy, and utilized to assay several commercially available G. lutea samples. Quantitative data obtained with the developed CE method are compared with HPLC results, and the advantages of each approach are discussed.