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Extraction of clenbuterol from urine using hydroxylated poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) monolith microextraction followed by high‐performance liquid chromatography determination
Author(s) -
Wen Yi,
Wang Ying,
Feng YuQi
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700321
Subject(s) - chromatography , detection limit , monolith , extraction (chemistry) , chemistry , monolithic hplc column , high performance liquid chromatography , glycidyl methacrylate , reproducibility , polymer , organic chemistry , polymerization , catalysis
A hydroxylated poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) (GMA‐ co ‐EDMA) monolithic capillary was prepared for polymer monolith microextraction (PMME). Coupled to HPLC with UV detection, this extraction medium was successfully applied to establish a simple and fast method for the analysis of clenbuterol (CLB) in urine. To obtain optimum extraction performance, the effects of pH value and ionic strength of the sample matrix on extraction efficiency were investigated. The linearity of the method was evaluated over a concentration range of 10–2000 ng/mL and the correlation coefficient ( R 2 value) was 0.9985. The detection limit and quantification limit were 2.3 and 7.7 ng/mL, respectively. Good reproducibility of the method was obtained, yielding the intra‐ and interday RSDs less than 5.1 and 9.1%, respectively. Moreover, the hydroxylated poly(GMA‐ co ‐EDMA) monolithic capillary exhibited good preparation reproducibility and long‐term extraction life. When applied to the determination of CLB in urine samples, an effective removal of interfering compounds was achieved and recoveries were in the range of 87.6–106%. The determination of CLB from one real sample including pretreatment, extraction, and analysis could be finished within 30 min.