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Solid‐phase microextraction for the determination of benzoylureas in orange juice using liquid chromatography combined with post‐column photochemically induced fluorimetry derivatization and fluorescence detection
Author(s) -
Parrilla Vázquez Piedad,
Mughari Ahmed R.,
Martínez Galera María
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700289
Subject(s) - chromatography , solid phase microextraction , orange juice , chemistry , derivatization , detection limit , high performance liquid chromatography , fluorescence spectroscopy , diflubenzuron , sample preparation , fluorescence , pesticide , gas chromatography–mass spectrometry , mass spectrometry , physics , food science , quantum mechanics , agronomy , biology
A solid‐phase microextraction (SPME) method has been developed for the determination of six benzoylureas (diflubenzuron, triflumuron, hexaflumuron, teflubenzuron, lufenuron, and flufenoxuron) in natural orange juice based on the direct immersion mode of a 60 μm polydimethylsiloxane/divinylbenzene fiber. An orange juice was obtained from blended, homogenized, and diluted ecological natural orange juice samples. An aliquot of 3 mL of a spiked sample was extracted under optimum SPME conditions. The determination of benzoylureas was carried out using HPLC combined with post‐column photochemically induced fluorimetry derivatization and fluorescence detection. The limits of quantification obtained in matrix were within the range of 0.02 to 0.04 mg/kg and these limits are lower than the maximum residue levels established in Spanish regulations for all pesticides in this study. Recoveries in juice samples ranged between 85 and 110% and relative standard deviations between 1.8 and 7.4%.