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Improved determination of 5‐fluorouracil and its prodrug tegafur in pharmaceuticals by large‐volume sample stacking in CE
Author(s) -
Yang Yanmin,
Liu Qian,
Tao Wenyan,
Nie Lihua,
Yao Shouzhuo
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700276
Subject(s) - prodrug , tegafur , chromatography , chemistry , volume (thermodynamics) , fluorouracil , sample preparation , stacking , organic chemistry , medicine , biochemistry , surgery , chemotherapy , physics , quantum mechanics
On‐line determination of the anti‐tumor drug 5‐fluorouracil (5‐FU) and its prodrug, tegafur (TF) was achieved for the first time by capillary electrophoresis with large‐volume sample stacking (CE–LVSS). The optimal electrophoretic buffer consisted of 30 mM phosphate buffer at pH 8.0. Without the LVSS procedure, the limits of detection (LOD) were 600.5 ng/mL and 771.4 ng/mL for 5‐FU and TF, respectively. With the LVSS procedure, the sensitivity was significantly improved by about two orders of magnitude (the LODs of 5‐FU and TF were decreased to 7.9 ng/mL and 6.5 ng/mL, respectively). The %RSD was less than 5%. This method compared favorably with other reported techniques and was applied successfully to the quantitative analysis of anti‐tumor drugs in commercial injection preparations. The results show that the method is simple, fast (less than 3 min), highly selective, and sensitive.