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Prediction of the location of stationary steady‐state zone positions in counterflow isotachophoresis performed under constant voltage in a vortex‐stabilized annular column
Author(s) -
Harrison Schurie L. M.,
Ivory Cornelius F.
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700243
Subject(s) - stack (abstract data type) , mechanics , constant (computer programming) , countercurrent exchange , chemistry , steady state (chemistry) , voltage , isotachophoresis , vortex , time constant , channel (broadcasting) , flow (mathematics) , control theory (sociology) , thermodynamics , physics , electrolyte , electrode , engineering , computer science , control (management) , quantum mechanics , artificial intelligence , electrical engineering , programming language
A theoretical model is presented and an analytical expression derived to predict the locations of stationary steady‐state zone positions in ITP as a function of current for a straight channel under a constant applied voltage. Stationary zones may form in the presence of a countercurrent flow whose average velocity falls between that of a pure leader zone and of a pure trailer zone. A comparison of model predictions with experimental data from an anionic system shows that the model is able to predict the location of protein zones with reasonable accuracy once the ITP stack has formed. This result implies that an ITP stack can be precisely directed by the operator to specific positions in a channel whence portions of the stack can be removed or redirected for further processing or analysis.