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A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography
Author(s) -
de Sousa João P. B.,
Bueno Paula C. P.,
Gregório Luiz E.,
da Silva Filho Ademar A.,
Furtado Niege A. J. C.,
de Sousa Márcia L.,
Bastos Jairo K.
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700228
Subject(s) - chromatography , chemistry , caffeic acid , ferulic acid , propolis , cinnamic acid , high performance liquid chromatography , elution , phenolic acid , extraction (chemistry) , ether , phenols , organic chemistry , food science , antioxidant
A sensitive and reliable RP‐HPLC method was developed using a C18 CLC‐ODS (M) – 4.6×250 mm 2 column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin‐4′‐methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2‐dimethyl‐6‐carboxyethenyl‐2 H ‐1‐benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83–533.33 μg/mL.