A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography | Zendy

de Sousa João P. B. | Zendy; Bueno Paula C. P. | Zendy; Gregório Luiz E. | Zendy; da Silva Filho Ademar A. | Zendy; Furtado Niege A. J. C. | Zendy; de Sousa Márcia L. | Zendy; Bastos Jairo K. | Zendy

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A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography

Author(s) -

de Sousa João P. B.,

Bueno Paula C. P.,

Gregório Luiz E.,

da Silva Filho Ademar A.,

Furtado Niege A. J. C.,

de Sousa Márcia L.,

Bastos Jairo K.

Publication year - 2007

Publication title -

journal of separation science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.72

H-Index - 102

eISSN - 1615-9314

pISSN - 1615-9306

DOI - 10.1002/jssc.200700228

Subject(s) - chromatography , chemistry , caffeic acid , ferulic acid , propolis , cinnamic acid , high performance liquid chromatography , elution , phenolic acid , extraction (chemistry) , ether , phenols , organic chemistry , food science , antioxidant

A sensitive and reliable RP‐HPLC method was developed using a C18 CLC‐ODS (M) – 4.6×250 mm 2 column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin‐4′‐methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2‐dimethyl‐6‐carboxyethenyl‐2 H ‐1‐benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83–533.33 μg/mL.

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