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Study of low‐molecular weight organic acids in maize roots under the stress of cadmium using capillary zone electrophoresis
Author(s) -
Guo BaoYuan,
Peng ZhenLei,
Han Fang,
Shan XiaoQuan,
Lin JinMing
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700207
Subject(s) - chemistry , cadmium , phthalate , salicylic acid , benzoic acid , chromatography , capillary electrophoresis , detection limit , phthalic acid , organic chemistry , biochemistry
An indirect CE method was developed for the study of low‐molecular weight organic acids (LMWOAs) in maize under the stress of cadmium. The influences of indirect reagents (phthalate, salicylic acid, and benzoic acid), coion concentration, and pH were studied. A buffer composed of 15 mmol/L benzoate and 0.2 mmol/L CTAB (pH 5.7) was used in the organic acid determination. The detection limit ranged between 0.5 and 6 μmol/L. The RSD ( n = 14) of the method was found to be in the range of 0.11–0.49% for migration time and 1.25–4.72% for peak area. In the maize roots, obvious increases of LMWOAs were observed when the plants were under the stress of cadmium and/or organic acids. The recovery of standard organic acids added in real samples ranged from 85 to 116%.