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Separation and evaluation of free radical‐scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC‐DPPH • method
Author(s) -
Pozharitskaya Olga N.,
Ivanova Svetlana A.,
Shikov Alexander N.,
Makarov Valery G.,
Galambosi Bertalan
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700178
Subject(s) - arbutin , gallic acid , chemistry , dpph , bergenin , ellagic acid , ascorbic acid , chromatography , hydroquinone , derivatization , skin whitening , phenols , organic chemistry , high performance liquid chromatography , antioxidant , polyphenol , food science , bioinformatics , active ingredient , biology
A new procedure has been developed to separate and quantify the free radical‐scavenging activity of individual compounds from green, brown, and black leaves of Bergenia crassifolia based on the combination of high performance TLC (HPTLC) with a diode array detector (DAD) and postchromatographic 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH • ) derivatization. Free gallic and ellagic acids, arbutin, hydroquinone, and bergenin in the B. crassifolia leaves' extracts were separated by HPTLC and identified. All compounds of the extract excluding bergenin were capable of scavenging DPPH∗ radicals. From the estimated ID 50 values, it can be seen that the increasing order of activity was gallic acid > arbutin > ellagic acid > hydroquinone > ascorbic acid. The antiradical activity of leaves of B. crassifolia is probably associated to the presence of phenol.