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One step purification of flavanone glycosides from Poncirus trifoliata by centrifugal partition chromatography
Author(s) -
Kim Chul Young,
Lee Hee Ju,
Lee Mi Kyeong,
Ahn MiJeong,
Kim Jinwoong
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700168
Subject(s) - naringin , chemistry , flavanone , chromatography , glycoside , high performance liquid chromatography , ethyl acetate , acetonitrile , organic chemistry , flavonoid , antioxidant
Flavanone glycosides were successfully separated from the crude extract of Poncirus trifoliata by preparative centrifugal partition chromatography with a two‐phase solvent system composed of ethyl acetate–acetonitrile–water (3:2:5, v/v/v). Naringin (50.0 mg), neoponcirin (16.8 mg), and poncirin (71.9 mg) were purified from the 524 mg crude extract in only one step. The purities of the isolated compounds were determined to be over 90% by HPLC analysis and their structures were elucidated by 1 H NMR, 13 C NMR, and ESI‐MS.