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Large‐scale separation of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by pH‐zone‐refining countercurrent chromatography
Author(s) -
Wang Xiao,
Geng Yanling,
Li Fuwei,
Liu Jianhua
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700167
Subject(s) - salvia miltiorrhiza , chromatography , chemistry , countercurrent chromatography , high performance liquid chromatography , solvent , aqueous solution , chloroform , extraction (chemistry) , organic chemistry , medicine , alternative medicine , pathology , traditional chinese medicine
pH‐Zone‐refining countercurrent chromatography was successfully applied to the separation of salvianolic acid B from the Chinese medicinal plant, Salvia miltiorrhiza Bunge, using a multilayer coil planet centrifuge. A 2.0 g quantity of sample was separated using the following two‐phase solvent system: methyl tert ‐butyl ether (MtBE)–water, 10 mM TFA in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by HPLC and ESI‐MS. The separation yielded 572 mg of the main component of salvianolic acid B with a purity of 94.1%.