Premium
Separation of three water‐soluble vitamins by poly(dimethylsiloxane) microchannel electrophoresis with electrochemical detection
Author(s) -
Li XiangYun,
Zhang QianLi,
Lian HongZhen,
Xu JingJuan,
Chen HongYuan
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700155
Subject(s) - chromatography , chemistry , ascorbic acid , detection limit , amperometry , capillary electrophoresis , pyridoxine , solid phase extraction , analyte , extraction (chemistry) , electrochemistry , electrode , biochemistry , food science
A method for rapid separation and sensitive determination of three water‐soluble vitamins, pyridoxine, ascorbic acid (VC), and p ‐aminobenzoic acid (PABA) has been developed by PDMS microchannel electrophoresis integrated with amperometric detection. After treatment of the microchip with oxygen plasma, the peak shapes of the three analytes were essentially improved. Pyridoxine, VC, and PABA were well separated within only 80 s in a running buffer of 20 mM borate solution (pH 8.5). Good linearity was obtained within the concentration range of 2–200 μM for the three water‐soluble vitamins. The detection limits were 1.0 μM for pyridoxine and VC, and 1.5 μM for PABA. The proposed method has been successfully applied to real human urine sample, without solid phase extraction, with recoveries of 80–122% for the three water‐soluble vitamins.