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Fluoxetine and norfluoxetine analysis by direct injection of human plasma in a column switching liquid chromatographic system
Author(s) -
SantosNeto Alvaro J.,
Fernandes Christian,
Rodrigues José C.,
Alves Claudete,
Lanças Fernando M.
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700153
Subject(s) - chromatography , chemistry , elution , quantitative analysis (chemistry) , extraction (chemistry) , matrix (chemical analysis) , sample preparation , analytical chemistry (journal)
A column switching LC method is presented for the analysis of fluoxetine (FLU) and norfluoxetine (NFLU) by direct injection of human plasma using a lab‐made restricted access media (RAM) column. A RAM‐BSA‐octadecyl silica (C‐18) column (40 mm×4.6 mm, 10 μm) is evaluated in both backflush and foreflush elution modes and coupled with a C‐18 lab‐made (50 mm×4.6 mm, 3 μm) analytical column in order to perform online sample preparation. Direct injection of 100 μL of plasma samples is possible with the developed approach. In addition, reduction of sample handling is obtained when compared with traditional liquid–liquid extraction (LLE) and SPE. The total analysis time is around 20 min. A LOQ of 15 ng/mL is achieved in a concentration range of 15–500 ng/mL, allowing the therapeutic drug monitoring of clinical samples. The precision values achieved are lower than 15% for all the evaluated points with adequate recovery and accuracy. Furthermore, no matrix interferences are found in the analysis and the proposed method shows to be an adequate alternative for analysis of FLU in plasma.