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A sensitive and selective RP‐HPLC method for simultaneous determination of picroside‐I and picroside‐II in rat plasma and its application in pharmacokinetics studies
Author(s) -
Lv Jing,
Pan Liyi,
Ye Yunhua,
Zhou Yawei
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700139
Subject(s) - chromatography , chemistry , calibration curve , analyte , detection limit , high performance liquid chromatography , acetonitrile
A sensitive and selective HPLC method with UV detection for the simultaneous determination of picroside‐I and picroside‐II (active components of total glycoside of Picrorhiza scrophulariiflora Pennell) was developed and validated in rat plasma. After simple deproteinization using acetonitrile, analysis was performed on an RP‐C18 column (250 mm×4.6 mm id, 5 μm) with a mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL/min used in a gradient elution program. The UV detection wavelength was set at 262 and 277 nm. Linear calibration curves were obtained in the concentration range of 0.10–50 μg/mL for picroside‐I and 0.25–200 μg/mL for picroside‐II. The lower limits of quantification were 0.1 and 0.25 μg/mL for picroside‐I and picroside‐II, respectively. The recoveries from spiked control samples were up to 80% for both picroside‐I and picroside‐II. Accuracy and precision of the validated method were both within the acceptable limits of <15% at three quality control concentrations. The analytes were stable after three freeze–thaw cycles. The method was successfully used to determine concentrations of picroside‐I and picroside‐II after intravenous administration of total glycoside of Picrorhiza scrophulariiflora Pennell to rats.