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Multiresidue HPLC analysis of ten quinolones in milk after solid phase extraction: Validation according to the European Union Decision 2002/657/EC
Author(s) -
Christodoulou Eleni A.,
Samanidou Victoria F.
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700129
Subject(s) - danofloxacin , flumequine , chromatography , chemistry , solid phase extraction , high performance liquid chromatography , enrofloxacin , european union , enoxacin , analyte , norfloxacin , detection limit , sample preparation , ciprofloxacin , biochemistry , business , economic policy , antibiotics
A rapid and sensitive analytical method was developed for the residue analysis of ten quinolones (enoxacin (ENO), ofloxacin (OFL), norfloxacin (NOR), ciprofloxacin (CIP), danofloxacin (DAN), enrofloxacin (ENR), sarafloxacin (SAR), oxolinic acid (OXO), nalidixic acid (NAL), and flumequine (FLU)) in cow's milk. The analytes were extracted from milk by a deproteinization step followed by a simple SPE cleanup procedure using LiChrolut RP‐18 Merck cartridges. Recoveries varied between 75 and 92%. HPLC separation was performed at 25°C using an ODS‐3 PerfectSil® Target (250×4 mm 2 ) 5 μm analytical column (MZ‐Analysentechnik, Germany). The mobile phase consisted of a mixture of TFA 0.1%–CH 3 CN–CH 3 OH, delivered by a gradient program at the flow rate of 1.2 mL/min. Elution of the ten analytes and the internal standard (caffeine, 7.5 ng/μL) was completed within 27 min. Column effluent was monitored using a photodiode array detector, set at 275 and 255 nm. The developed method was validated according to the criteria of Commission Decision 2002/657/EC. The LODs of the specific method of quinolones' determination in milk varied between 1.5 and 6.8 ng/μL.