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Sodium maleopimaric acid as pseudostationary phase for chiral separations of amino acid derivatives by capillary micellar electrokinetic chromatography
Author(s) -
Wang Hengshan,
Zhao Shulin,
He Min,
Zhao Zhicai,
Pan Yingming,
Liang Qian
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700128
Subject(s) - micellar electrokinetic chromatography , chemistry , chromatography , electrokinetic phenomena , chiral stationary phase , phase (matter) , capillary electrophoresis , amino acid , high performance liquid chromatography , organic chemistry , biochemistry
A new type of chiral surfactant, sodium maleopimaric acid (SMA), was synthesized, and employed for the enantioselective micellar electrokinetic chromatographic (MEKC) separation of amino acid enantiomers derivatized with naphthalene‐2,3‐dicarboxaldehyde (NDA‐ D / L ‐AAs). The effect of the surfactant concentration, type and concentration of the BGE, and buffer pH on the resolution was studied, and optimized conditions were used to evaluate the ability of this new surfactant to perform chiral separations toward NDA‐ D / L ‐AAs by MEKC. Enantiomeric separations of NDA‐ D / L ‐AAs were achieved with a running buffer consisting of 100 mM borate (pH 9.5) and 20 mM SMA in a 58.5 cm length×50 μm id capillary. Under the conditions selected, two pairs of tested amino acid enantiomers including NDA‐ D / L ‐trptophan (Trp) and NDA‐ D / L ‐kynurenine (Kyn) were resolved.