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A strategy to develop fast RP‐HPLC methods using monolithic silica columns
Author(s) -
El Deeb Sami,
Preu Lutz,
Wätzig Hermann
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700092
Subject(s) - monolithic hplc column , column (typography) , transferability , volumetric flow rate , computer science , chromatography , high performance liquid chromatography , materials science , process engineering , flow (mathematics) , chemistry , mathematics , engineering , telecommunications , physics , geometry , logit , frame (networking) , quantum mechanics , machine learning
Since the appearance of monolithic silica, much work has been done describing the properties of monolithic silica columns. Meanwhile the transferability of analytical methods from conventional to monolithic silica columns has been intensively investigated [1–5]. RP HPLC method development strategies for conventional columns should be updated or scaled to meet the higher performing monolithic column technology. Because of the high permeability of monolithic silica columns it should be possible to decrease the time for method development by applying high isocratic flow rates. Here we suggest a clear strategy for method development using monolithic columns. The strategy will be applicable for various sample compositions, e. g. , acidic, basic, or neutral. The applicability of monolithic columns for especially complex separations of basic mixtures without the need of using a highly basic mobile phase that harms the column will be pointed out in this work. This work will describe in detail the actual method development process. For better understanding of our strategy, the influence of flow rate, column length, mobile phase composition, pH, and temperature will be discussed. Details about the application of a flow program will be mentioned.