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Preparation and chromatographic properties of a multimodal chiral stationary phase based on phenyl‐carbamate‐propyl‐β‐CD for HPLC
Author(s) -
Si Ahmed Kahina,
Tazerouti Fairouz,
BadjahHadjAhmed Ahmed Yacine,
Meklati Brahim Youcef
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700047
Subject(s) - chiral stationary phase , chromatography , high performance liquid chromatography , chemistry , carbamate , stationary phase , reversed phase chromatography , phase (matter) , organic chemistry
A phenylcarbamate derivative of 2‐hydroxypropyl‐β‐CD bonded stationary phase was prepared by a previously described method. Its enantiomeric recognition abilities were evaluated as chiral stationary phase (CSP) in normal, polar organic and RP conditions by HPLC. The relevant structural features of the prepared stationary phase which make it an effective chiral selector are discussed. This material seems to have an excellent enantioselectivity for a variety of racemic analytes in the three modes. Hence it can be considered a highly effective multimodal column. Retention factor ( k ), selectivity (α) and resolution ( R s ) were the chosen parameters to describe the column performance. Optimization of these separations was discussed in terms of mobile phase composition, flow rate and structural patterns of the injected analytes.