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Stability studies of stationary phases from poly(methyltetradecylsiloxane) sorbed and immobilized onto metalized and unmodified silicas
Author(s) -
Faria Anizio M.,
Tonhi Edivan,
Collins Kenneth E.,
Collins Carol H.
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700027
Subject(s) - thermal stability , stationary phase , chemistry , chromatography , chemical stability , silica gel , high performance liquid chromatography , phase (matter) , chemical engineering , materials science , organic chemistry , engineering
Stationary phases for RP‐HPLC were prepared from metalized (titanized and zirconized) and unmodified silica particles using sorbed and immobilized poly(methyltetradecylsiloxane) (PMTDS). Different immobilization procedures, such as gamma irradiation and thermal treatments, were used for the preparation of the immobilized PMTDS phases. The stabilities of these stationary phases were evaluated by passing alkaline (pH 10) mobile phase through 60 mm×3.9 mm columns of the different phases, with periodic tests to evaluate chromatographic performance. The results show that higher stabilities were obtained with stationary phases based on PMTDS immobilized on zirconized silica, these phases being 50% more stable than their titanized silica counterparts and 400% more stable than those based on unmodified silica. These supports provide higher chemical stability to the laboratory‐made stationary phases, when compared with chemically bonded silica‐based phases.