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Orthogonal array design for the optimization of supercritical fluid extraction of baicalin from roots of Scutellaria baicalensis Georgi
Author(s) -
Chang Yiling,
Liu Ben,
Shen Bo
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700020
Subject(s) - baicalin , supercritical fluid extraction , supercritical fluid , chromatography , extraction (chemistry) , scutellaria baicalensis , supercritical carbon dioxide , chemistry , supercritical fluid chromatography , high performance liquid chromatography , methanol , organic chemistry , medicine , alternative medicine , traditional chinese medicine , pathology
Abstract Supercritical fluid was used to extract baicalin from the roots of Scutellaria baicalensis Georgi. An orthogonal array design (OAD), OA 9 (3 4 ), was employed as a chemometric method for the optimization of the supercritical fluid extraction of baicalin from the herbal medicine. Four parameters, namely, modifiers, temperature and pressure of supercritical fluid, and the dynamic extraction time, were studied and optimized by a three‐level OAD in which the interactions between the parameters were neglected. Determinations of the extracts were performed by high‐performance liquid chromatography. The effects of parameters were studied using analysis of variance. The results showed that selection of the modifier was the main factor in attaining higher yields of baicalin. While the other three factors had some effect on the extraction of the compound, the effect was much less than that of the modifiers. 1,2‐Propanediol‐modified supercritical fluid was more effective than 95% ethanol‐modified supercritical carbon dioxide or methanol‐modified supercritical carbon dioxide for the extraction of baicalin from the solid matrix. Finally, experimental conditions were proposed which can provide the highest extraction yield with respect to the considered factors.