Premium
Simultaneous characterization of isoflavonoids and astragalosides in two Astragalus species by high‐performance liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry
Author(s) -
Zhang Xi,
Xiao HongBin,
Xue XingYa,
Sun YanGuo,
Liang XinMiao
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700014
Subject(s) - atmospheric pressure chemical ionization , chemistry , chromatography , high performance liquid chromatography , astragalus , mass spectrometry , tandem mass spectrometry , chemical ionization , astragaloside , analytical chemistry (journal) , ionization , ion , organic chemistry , medicine , alternative medicine , traditional chinese medicine , pathology
Abstract A method was developed for the simultaneous identification of astragalosides (AGs) and isoflavonoids (IFs) in the roots of Astragalus membranaceus and Astragalus mongholicus by HPLC coupled with atmospheric pressure chemical ionization MS/MS (HPLC‐APCI‐MS/MS). Diagnostic fragment ions of AGs and different group of IFs were obtained with one AG and eight IF standards analyzed by CID‐MS, which were adopted as characteristic MS/MS fingerprints for further identification of these compounds in the two Astragalus species by using HPLC‐APCI‐MS/MS. A total of 20 IFs and 10 AGs were identified or tentatively identified. Among them, six IFs were detected in A. membranaceus for the first time and five IFs were firstly identified in A. mongholicus . The results indicate that HPLC‐APCI‐MS/MS is a powerful tool for the simultaneous characterization of IFs and AGs in complex matrix.