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Fast gas chromatography‐full scan quadrupole mass spectrometry for the determination of allergens in fragrances
Author(s) -
Mondello Luigi,
Sciarrone Danilo,
Casilli Alessandro,
Tranchida Peter Quinto,
Dugo Paola,
Dugo Giovanni
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600541
Subject(s) - chemistry , chromatography , mass spectrometry , analyte , european union , retention time , cosmetics , kovats retention index , quadrupole mass analyzer , analytical chemistry (journal) , gas chromatography , organic chemistry , business , economic policy
The Scientific Committee for Cosmetics and NonFood Products in the 7th Amendment to the European Cosmetics Directive established 26 fragrance components, widely used in cosmetic products, as being responsible for a series of contact allergies (Directive 2003/15/EC, Official Journal of the European Union, L66/26, 11.3.2003). The regulation foresees that any allergen, present in excess of 100 mg/kg in rinse‐off and of 10 mg/kg in leave‐on formulations, must be reported on the label of the product. The present research reports a fast GC‐full scan quadrupole mass spectrometric method (under 5 min) for the qualitative/quantitative analysis of allergens in perfumes. Reliable peak identification was achieved through a twin‐filtered MS library matching procedure, considering a minimum degree of spectral similarity (90%) and retention data (a linear retention index window was applied). Peak quantification was carried out by using a specific extracted ion. In case a suspected allergen fell within its retention time window but presented a low degree of spectral purity (< 90%), analyte determination was achieved by using three extracted ions (one quantifier and two qualifiers). The fast GC‐MS method was validated in terms of intraday retention time and peak area precision, LODs and LOQs, and method linearity. Finally, peak skewing was also evaluated and was within more than acceptable limits.