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Surface modified polypropylene pipette tips packed with a monolithic plug of adsorbent for high‐throughput sample preparation
Author(s) -
Altun Zeki,
Hjelmström Anette,
AbdelRehim Mohamed,
Blomberg Lars G.
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600523
Subject(s) - monolith , polypropylene , pipette , surface modification , materials science , sample preparation , chromatography , chemical engineering , polymer , contact angle , polymerization , adsorption , methacrylate , composite material , chemistry , organic chemistry , engineering , catalysis
UV‐initiated poly(butyl methacrylate‐ethylene glycol dimethacrylate) porous polymer monoliths were prepared in situ in polypropylene‐based pipette tips for high‐throughput sample preparation. Prior to the in situ polymerization, the surface of the PP tips was modified. In this work, two different surface modification approaches were tested for this purpose. First the photoinitiator benzophenone was used to generate radicals at the surface of PP by hydrogen abstraction. In the second modification approach, a thin layer of a polymer was directly grafted to the surface. The effect of surface modification was measured by contact angle measurements of a drop of water at the surface. As a result of the surface modification, scan electron microscopy images indicate a covalent attachment of the monolith to the wall of the pipette tip. Pipette tips modified with 5% BP in methanol and packed with a plug of monolith were further evaluated for high‐throughput sample preparation. Using a liquid handling system, the extraction performance of packed pipette tips was tested for the analysis of ropivacaine in plasma samples. The recovery and reproducibility results were in accordance with internationally accepted criteria for qualitative and quantitative analysis of the test substance, ropivacaine.