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Simultaneous determination of phosphonate, phosphate, and diphosphate by capillary electrophoresis using in‐capillary complexation with Mo(VI)
Author(s) -
Himeno Sadayuki,
Inazuma Naoko,
Kitano Eri
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600501
Subject(s) - phosphonate , chemistry , capillary electrophoresis , phosphate , chromatography , calibration curve , phosphate buffered saline , detection limit , malonate , analytical chemistry (journal) , organic chemistry
This study describes the simultaneous determination of phosphonate, phosphate, and diphosphate by CE with direct UV detection, based on in‐capillary complexation with Mo(VI). When a mixture of phosphonate, phosphate, and diphosphate was injected into a capillary containing 3.0 mM Mo(VI), 0.05 M malonate buffer (pH 3.0) and 45% v/v CH 3 CN, three well‐defined peaks, due to the migration of the corresponding polyoxomolybdate anions, were separated. The respective calibration graphs were linear in the concentration range of 2×10 –6 –2×10 –4 M for phosphonate, 1×10 –6 –5×10 –5 M for phosphate, and 1×10 –6 –2×10 –4 M for diphosphate; the correlation coefficients were better than 0.9990. The present CE method is successfully applied to the simultaneous determination of phosphonate, phosphate, and diphosphate in tap water.