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Multiphoton excitation fluorescence: A versatile detection method for capillary electrophoresis
Author(s) -
Du Wei,
Chen Sheng,
Xu Youzhi,
Chen Zhe,
Luo Qingming,
Liu BiFeng
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600477
Subject(s) - bioanalysis , capillary electrophoresis , excitation , fluorescence , field (mathematics) , detector , laser induced fluorescence , chemistry , nanotechnology , chromatography , materials science , optics , physics , mathematics , quantum mechanics , pure mathematics
Multiphoton excitation is a relatively old concept in quantum optics. But using multiphoton excitation fluorescence (MPEF) for bioanalysis is still in its infancy. Recently, MPEF has been introduced into the microseparation field, particularly CE, as a novel detection method. In this paper, MPEF detection for CE is reviewed, including MPEF fundamentals, approaches to achieving MPEF, detector configurations and applications in biological and environmental analyses. Emphasis will be placed on some recent advances of CE‐MPEF in our laboratory. Challenges and future prospects are also discussed.