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Identification of unknown compounds on the basis of retention index data in comprehensive two‐dimensional gas chromatography
Author(s) -
Pang Tao,
Zhu Shukui,
Lu Xin,
Xu Guowang
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600471
Subject(s) - kovats retention index , gas chromatography , chromatography , identification (biology) , chemistry , index (typography) , computer science , botany , world wide web , biology
The identification of unknown compounds in complex samples is very difficult. Comprehensive two‐dimensional gas chromatography (GC×GC) provides very good resolution and improved identification reliability. Mass spectrometry is a powerful identification tool and retention index data are another good approach to this end. In this study, a second‐order polynomial was used to calculate retention index data based on n ‐alkanes beyond the region of the ‘isovolatile’ curve in GC×GC, and the results in the 2nd dimension were validated by using the same stationary phase column in one‐dimensional GC. To test the usefulness of the method, volatile compounds in a tobacco leaf extract fraction were analyzed using GC×GC, and 60 compounds were identified on the basis of their retention indices.