Premium
Preparation and chiral recognition ability of crosslinked beads of polysaccharide derivatives
Author(s) -
Ikai Tomoyuki,
Yamamoto Chiyo,
Kamigaito Masami,
Okamoto Yoshio
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600438
Subject(s) - cellulose , chemistry , high performance liquid chromatography , chromatography , polysaccharide , derivative (finance) , chloroform , silica gel , column chromatography , polymer chemistry , organic chemistry , financial economics , economics
The spherical beads consisting of cellulose 3,5‐dimethylphenylcarbamate with partial hydroxyl groups were prepared to be used as chiral packing materials (CPMs) for HPLC. The beads were obtained without using macroporous silica gel, which is usually used as the support of the CPMs based on the polysaccharide derivatives. After the crosslinking in the bead with diisocyanates, such as 4,4′‐diphenylmethane diisocyanate (MDI), 4,4′‐dibenzyl diisocyanate (DBDI), tolylene‐2,4‐diisocyanate (TDI), and m ‐xylylene diisocyanate (XDI), the obtained beads were packed into an HPLC column. As the content of the hydroxyl groups of the cellulose derivatives decreased, the obtained CPM exhibited a higher chiral recognition ability. The beads possessed a higher loading capacity than the CPM prepared by coating the cellulose derivative on silica gel. The crosslinked beads could be used with the eluent containing chloroform. The amylose derivative beads were also prepared as a CPM for chiral HPLC.