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Application of liquid chromatography coupled with electrospray ionization time‐of‐flight mass spectrometry for screening and quantitative analysis of C 21 steroids in the roots and rhizomes of Cynanchum paniculatum
Author(s) -
Dou Jing,
Li Ping,
Song Yue,
Qi LianWen,
Bi ZhiMing
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600434
Subject(s) - chromatography , chemistry , mass spectrometry , electrospray ionization , rhizome , electrospray , acetonitrile , ion suppression in liquid chromatography–mass spectrometry , time of flight mass spectrometry , high performance liquid chromatography , analytical chemistry (journal) , ionization , liquid chromatography–mass spectrometry , ion , ecology , organic chemistry , biology
A liquid chromatography‐mass spectrometry method is presented for the quantification of C 21 steroids in the roots and rhizomes of Cynanchum paniculatum . Eight C 21 steroids, including five steroidal aglycones and three steroidal glycosides, were simultaneously analyzed by liquid chromatography coupled with electrospray ionization time‐of‐flight mass spectrometry. The extracted ion current chromatograms were extracted from the total ion current chromatogram using characteristic ions produced by target compounds for peak determination. Chromatographic separation was achieved on a C 18 reversed‐phase column within 60 min, using an acetonitrile/water gradient. For comparision, six C. paniculatum samples from different locations were investigated by the established method, and the results indicated that the different geographical origin significantly influenced the C 21 steroid composition. The method was observed to have the necessary sensitivity, selectivity, precision, and accuracy, and to be suitable for quality control of herbal medicines and their preparations.