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Determination of ( E )‐10‐hydroxy‐2‐decenoic acid content in pure royal jelly: A comparison between a new CZE method and HPLC
Author(s) -
Ferioli Federico,
Marcazzan Gian Luigi,
Caboni Maria Fiorenza
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600416
Subject(s) - royal jelly , repeatability , chromatography , chemistry , high performance liquid chromatography , linear relationship , quantitative analysis (chemistry) , significant difference , food science , statistics , mathematics
A new CZE method was developed and compared with HPLC for the determination of ( E )‐10‐hydroxy‐2‐decenoic acid (10‐HDA) in royal jelly (RJ) samples of different geographical origin. The results obtained with the CZE method were highly correlated with those of HPLC ( p <0.01). Under optimized conditions, CZE employed minimal amounts of 50 mM tetraborate buffer as BGE, without the addition of organic solvents, EOF or pH modifiers. The CZE method showed a wide linear response range (0.006–0.808 mg 10‐HDA/mL), a good sensitivity (LOD and LOQ were 0.002 and 0.004 mg/mL, respectively) and a satisfactory instrumental repeatability with respect to migration time and peak area (RSD% less than 1.0 and 2.0% on migration time for intra‐ and interday assay, respectively and less than 2.0 and for 4.0% on peak area for intra‐ and interday assay, respectively). The 10‐HDA content in RJ ranged from 0.8 to 3.2 g/100 g of RJ and a significant difference ( p <0.05) was found between the Italian and extra‐European average values: 2.5 and 1.6 g/100 g of RJ, respectively, according to the CZE data. The possibility of application of CZE for routine analyses on RJ and RJ based products to verify their authenticity is highlighted here.