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Quantitative determination of haloperidol in tablets by high performance thin‐layer chromatography
Author(s) -
Mennickent Sigrid,
Pino Loreto,
Vega Mario,
Godoy Carmen Gloria,
de Diego Marta
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600408
Subject(s) - chromatography , haloperidol , thin layer chromatography , high performance thin layer chromatography , chemistry , high performance liquid chromatography , medicine , dopamine
A densitometric high performance thin‐layer chromatography (HPTLC) method was developed and validated for the quantitative analysis of haloperidol in tablets. Chromatographic separation was achieved on precoated silica gel F 254 HPTLC plates using a mixture of acetone/chloroform/ n ‐butanol/acetic acid glacial/water (5:10:10:2.5:2.5 v/v/v/v/v) as the mobile phase. Quantitative analysis was carried out at a wavelength of 254 nm. The method was linear in the 10–100 ng/μL range, with a determination coefficient of 0.999. The coefficients of variation for precision were not higher than 2.35%. The detection limit was 0.89 ng/μL, and the quantification limit was 2.71 ng/μL. The accuracy ranged from 97.76 to 100.33%, with a CV not higher than 4.50%. This method was successfully applied to quantify haloperidol in real pharmaceutical samples, including the comparison with HPLC measurements. The method was fast, specific, with a good precision and accuracy for the quantitative determination of haloperidol in tablets.