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Fabrication of a poly(styrene–octadecene–divinylbenzene) monolithic column and its comparison with a poly(styrene–divinylbenzene) monolithic column for the separation of proteins
Author(s) -
Gu Congying,
Lin Li,
Chen Xiaodong,
Jia Jinping,
Ren Jicun,
Fang Nenghu
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600397
Subject(s) - divinylbenzene , monolith , styrene , monolithic hplc column , polymerization , materials science , monomer , theoretical plate , chromatography , polymer chemistry , chemical engineering , resolution (logic) , copolymer , polymer , chemistry , capillary action , organic chemistry , catalysis , composite material , engineering , artificial intelligence , computer science
In this paper, a poly(styrene‐octadecene‐divinylbenzene) (PS‐OD‐DVB) monolithic column was prepared in one step by introducing a C 18 carbon chain as monomer. N,N ‐Dimethylformamide and decanol served as porogens to make a homogeneous polymerization mixture in a fused silica capillary (320 μm inner diameter). Its physical and chromatographic properties were compared with those of poly(styrene‐divinylbenzene) (PS‐DVB) monolithic column, which was also fabricated by in‐situ polymerization in a fused silica capillary with the same inner diameter. Six standard proteins were used to evaluate the columns and their potential application for the separation of human hemoglobin was also discussed. It was shown that the PS‐OD‐DVB and PS‐DVB monoliths appeared to have similar efficiency for rapid separation of six proteins within 3.5 min. The PS‐OD‐DVB monolith was found to have higher loading capacity and higher resolution for the separation of α and β chains of hemoglobin because of the introduction of C 18 carbon chains, and shows great potential for the separation of bio‐macromolecules.